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June 8, 2026Open Access

"Rp-HPLC Method Development and Validation for Simultaneous Estimation of Linezolid and Daptomycin and Their Impurities."

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Authors

JDJyosna B.Tayade, Dr. Madhuri A.Theng, Mr. Amol G.Jadhao, Dr. Ashutosh K. Dash

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Overview

Randomized trial demonstrates a validated RP-HPLC method for estimating Linezolid and Daptomycin in pharmaceuticals, implying its utility in quality control.

Key Points

  • The study aimed to develop and validate a gradient RP-HPLC method for estimating Linezolid and Daptomycin and their impurities in pharmaceutical formulations.
  • Developed a gradient RP-HPLC method using a C18 column with phosphate buffer and acetonitrile as mobile phase.
  • Validation followed ICH Q2(R1) guidelines for specificity, linearity, accuracy, and precision, including recovery and degradation studies.
  • Assessed marketed formulations to demonstrate routine applicability in quality control.
  • Excellent linearity for both drugs over 10–100 μg/mL with correlation coefficients > 0.999.
  • Percentage recovery between 98–102%, with precision (%RSD) values below 2%.
  • Forced degradation studies confirmed stability-indicating capability, effectively separating degradation products.

Cite This Study

Jyosna B.Tayade, Dr. Madhuri A.Theng, Mr. Amol G.Jadhao, Dr. Ashutosh K. Dash (2026) studied this question.

synapsesocial.com/papers/6a265ca8ad53cfb9357c5f44https://doi.org/10.5281/zenodo.20568146
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