A new concept of photocrosslinking mechanism is developed to construct a 3D cell culture matrix based on a photo-uncaged-thiol Michael addition. This first-class approach not only provides highly cytocompatible gelation in a spatiotemporally controlled manner, but also controls gel stiffness via appropriate light dose, signaling desired cues to live cells and thus important to bioengineering and regenerative medicine.
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Liu et al. (2014) studied this question.
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