Key result
An aptamer-based viability impedimetric sensor successfully detected viable vaccinia virus particles down to 60 virions per microliter and distinguished them from nonviable viruses.
The developed aptasensor provides a highly selective, label-free electrochemical assay for detecting viable viruses.
The development of aptamer-based viability impedimetric sensor for viruses (AptaVISens-V) is presented. Highly specific DNA aptamers to intact vaccinia virus were selected using cell-SELEX technique and integrated into impedimetric sensors via self-assembly onto a gold microelectrode. Remarkably, this aptasensor is highly selective and can successfully detect viable vaccinia virus particles (down to 60 virions in a microliter) and distinguish them from nonviable viruses in a label-free electrochemical assay format. It also opens a new venue for the development of a variety of viability sensors for detection of many microorganisms and spores.
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Labib et al. (2012) studied Vaccinia virus detection. Aptamer-based viability impedimetric sensor (AptaVISens-V) vs. Nonviable viruses was evaluated on Detection of viable vaccinia virus particles. An aptamer-based viability impedimetric sensor successfully detected viable vaccinia virus particles down to 60 virions per microliter and distinguished them from nonviable viruses.
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