Establishment of Callus Regeneration System for Acer ginnala Maxim and Determination of Gallic Acid in Callus

A reproducible system for callus induction of Acer ginnala Maxim is described. The system involves mature seeds sprouted on MS medium supplemented with 0.1 mg·L⁻¹6-BA, the stems of young seedling as explants, and calli easily induced on woody plant medium (WPM) supplemented with 0.002-0.01 mg·L⁻¹ TDZ and 0.1 mg·L⁻¹6-BA 3 weeks following induction. The frequency of callus induction can be as high as 98.0%. On WPM containing 0.01 mg·L⁻¹ TDZ and 0.1 mg·L⁻¹6-BA, 42% of calli differentiated, and the mean number of buds per piece of callus was about 10. The buds developed roots on WPM medium with 0.3 mg·L⁻¹ IBA and formed plantlets, 89% of which survived on transplantation to the greenhouse. Methods to extract and determine gallic acid from callus were established and showed 2.8% content of gallic acid in bottles of green callus subcultured twice.