Key result
Homozygous eIF2alpha mutant mouse embryo fibroblasts with a Ser51 to Ala mutation were resistant to the apoptotic effects of dsRNA, TNF-alpha, and serum deprivation.
Population
Homozygous eIF2alpha mutant mouse embryo fibroblasts (Ser51 replaced with Ala) and wild-type cells
Comparison
Gene targeting to replace wild-type Ser51 allele… vs Wild-type cells
Design
Preclinical
Authors
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eIF2α phosphorylation may regulate apoptosis in stressed cells; leaves open its role in human cardiovascular disease models.
Phosphorylation of eIF2alpha at Ser51 is a fundamental death signal sufficient to induce apoptosis, though its ultimate effect depends on coincident signaling events.
Scheuner et al. (2006) studied Apoptosis and cell stress signaling. Gene targeting to replace wild-type Ser(51) allele with Ala in eIF2alpha gene vs. Wild-type cells was evaluated on Apoptosis and caspase 3 activation. Homozygous eIF2alpha mutant mouse embryo fibroblasts with a Ser51 to Ala mutation were resistant to the apoptotic effects of dsRNA, TNF-alpha, and serum deprivation.
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