GC-MS Profiling of Phytoconstituents from Bauhinia variegata Linn. bark: Identification of Triterpenoids and Bioactive Secondary Metabolites.

ABSTRACTThe identification of bioactive secondary metabolites is crucial for thepharmacological validation of medicinal plants. In this study, an Agilent7890A/5975C GC-MS system equipped with a DB-5MS column was employed toprofile Bauhinia variegata Linn. bark extract. The oven program ranged from 60 °Cto 300 °C, and compounds were identified via the NIST 4.0 library. Chromatographicanalysis resolved 21 distinct phytoconstituents. The major component was identifiedas 9,19-Cyclolanost-24-en-3-ol (12.29%), followed by significant concentrations ofpentacyclic triterpenoids, including alpha-amyrin (6.85%) and beta-amyrin (1.86%).The method effectively separated isomeric triterpenes and late-eluting sterols likegamma-sitosterol (3.29%). The results demonstrate a diverse chemical profile rich inanti-inflammatory markers, providing a standardized chemical baseline for futuretherapeutic investigations.