Synapse
⌘+K
Synapse
PulseExploreClubsResearchersJournals
Instagram
HomeClubsExplore
June 13, 2026Frontiers in ImmunologyOpen Access

Versatile electroporation protocols enable reproducible CRISPR-RNP delivery across multiple primary mouse cells of the hematopoietic lineage

View Full Paper
Ask AI
Bookmark
Share

Authors

JOJu Hee OhLYLucy YangETErin Tanaka

Discussion

Loading...

Member takes

Overview

Randomized trial shows efficient gene editing in hematopoietic cells, suggesting a versatile approach for genetic studies.

Key Points

  • The goal is to optimize CRISPR-Cas9 electroporation protocols for effective genetic engineering of primary mouse hematopoietic cells.
  • Utilized the ExPERT platform to optimize electroporation workflows across three primary hematopoietic cell types.
  • Assessed cell viability and gene knockout efficiency in activated OT-I CD8 + T cells, BMDMs, and HSPCs.
  • Evaluated sensitivity to electroporation parameters based on cell activation state.
  • Achieved high-efficiency RNP delivery in activated OT-I CD8 + T cells with minimal impact on cell viability.
  • BMDMs demonstrated robust RNP delivery and maintained high viability and differentiation post-electroporation.
  • Consistent RNP delivery and reproducible gene knockout were observed in HSPCs with the optimized protocol.

Cite This Study

Oh et al. (2026) studied this question.

synapsesocial.com/papers/6a2cf2a9faef96ed7f055673https://doi.org/10.3389/fimmu.2026.1820963
View Full Paper
Ask AI
Bookmark
Share

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Miniaturized scalable arrayed CRISPR screening in primary cells enables discovery at the single donor resolution2025 · 1 citations
  2. 2Lentiviral CRISPR/Cas9-Mediated Genome Editing for the Study of Hematopoietic Cells in Disease Models2019 · 14 citations
  3. 3Optimized CRISPR/Cas9 Electroporation and Single Cell Cloning Protocol for Generating Pure Cellular Models in Human Immortalized Myoblasts2025
  4. 4Exploring non-viral methods for the delivery of CRISPR-Cas ribonucleoprotein to hematopoietic stem cells2024 · 16 citations
  5. 5Safe and Efficient CRISPR Genome Editing of Primary Human T Cells Using a Droplet‐Based Cell Mechanoporation Platform2026 · 1 citations