Synapse
⌘+K
Synapse
PulseExploreJournal ClubResearchersJournals
Instagram
HomeJournal ClubExplore
June 13, 2026Cell & BioscienceOpen Access

5′-end uridine-derived base modification for siRNAs potency reinforcement

View Full Paper
Ask AI
Bookmark
Share

Authors

YYYuqi YaoXQXinming QiRHRuimin Huang

Discussion

Loading...

Member takes

Overview

Randomized trial evaluated siRNA modifications for gene silencing, revealing improved potency and safety.

Key Points

  • This research aims to enhance the potency of siRNAs by modifying the 5'-end nucleotide using uridine-derived changes.
  • Conducted structural analysis of the 5'-end binding pocket of the RISC complex.
  • Designed and screened novel uridine-derived modifications including 001U-018U and vs30U.
  • Tested modified siRNA binding activity in vitro using HepG2 cells and in vivo in mice.
  • Modified siRNAs showed significantly improved gene silencing activity compared to unmodified controls (p<0.05).
  • Safety evaluations indicated no detectable cytotoxicity or genomic insertion risks from modified nucleosides.

Cite This Study

Yao et al. (2026) studied this question.

synapsesocial.com/papers/6a2cf3b4faef96ed7f056211https://doi.org/10.1186/s13578-026-01586-z
View Full Paper
Ask AI
Bookmark
Share