Review explores epigenetic roles of DNA methylation in glioblastoma, indicating potential for personalized therapies.
Key Points
This review aims to explore the emerging roles of DNA methylation in glioblastoma and the applications of single-cell epigenomics.
Analyzed DNA methylation alterations in IDH-wildtype glioblastoma using single-cell bisulfite sequencing and reduced representation bisulfite sequencing.
Integrated data from single-cell methylation with transcriptomics and proteomics to understand tumor dynamics.
Addressed challenges in cost, data interpretation, and large-scale integration of single-cell epigenomic data.
Identified that promoter hypermethylation silences key tumor suppressor genes.
Revealed global hypomethylation contributes to genomic instability and tumor progression.
Demonstrated that epigenetic diversity influences subclonal expansion and therapy resistance.