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June 14, 2026Journal of Food Composition and AnalysisOpen Access

Colorimetric Lyophilized Loop-Mediated Isothermal Amplification for Rapid Detection of Streptococcus suis in raw pork samples

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Authors

LKLikhitphorn KhamaiRGRatchadakorn GuntalaSYSumed Yadoung

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Overview

Randomized trial evaluates a colorimetric lyophilized assay for detecting Streptococcus suis in raw pork, suggesting effective field deployment.

Key Points

  • The aim is to develop a rapid, stable assay for detecting Streptococcus suis in pork samples to facilitate on-site testing.
  • Developed a colorimetric lyophilized LAMP assay for S. suis detection.
  • Assay performed at 65 °C for 60 minutes using a pH-sensitive dye.
  • Evaluated with 111 raw pork samples for diagnostic performance.
  • The lyophilized assay demonstrated high analytical sensitivity and specificity without cross-reactivity to 12 bacteria.
  • Reagents remained functional for at least 30 days at ambient temperatures.
  • Successfully validated the assay with raw pork samples with robust results.

Cite This Study

Khamai et al. (2026) studied this question.

synapsesocial.com/papers/6a2e4608b1cc60ccdea8ae36https://doi.org/10.1016/j.jfca.2026.109311
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