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June 14, 2026Biochemical and Biophysical Research CommunicationsOpen Access

DNMT1-regulated hypomethylation enhances miR-300-3p to promote ferroptosis in Ang II-induced cardiac hypertrophy by suppressing ACOX1.

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Why the study?

Cardiac hypertrophy is a key process in remodeling and heart failure progression, but the specific regulatory involvement of microRNAs remains incompletely understood.

Population

Preclinical model of Angiotensin II (Ang II)-induced cardiac hypertrophy

Comparison

Modulation of miR-300-3p and ACOX1 expression vs Control models

Design

Preclinical

Key result

DNMT1-regulated promoter hypomethylation enhances miR-300-3p expression, which suppresses ACOX1 and promotes ferroptosis in Ang II-induced cardiac hypertrophy.

Authors

XLXiaojuan LiYWYanjia WangZYZiyao Yang

Discussion

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Overview

Should not change hypertrophy management; hypothesis-generating for miR-300-3p/ACOX1/GPX4 targeting in remodeling.

Key Points

  • The aim is to explore how miR-300-3p influences cardiac hypertrophy through the ACOX1/GPX4 pathway.
  • In vitro assays to assess miR-300-3p effects on CH in response to angiotensin II (Ang II) stimulation.
  • Evaluation of ACOX1 expression changes with overexpression experiments.
  • Investigation of promoter hypomethylation effects mediated by DNMT1.
  • miR-300-3p promotes cardiac hypertrophy by targeting ACOX1, leading to increased ferroptosis.
  • Overexpression of ACOX1 significantly reduced hypertrophic responses.
  • DNMT1-mediated hypomethylation elevated miR-300-3p, enhancing Ang II-induced hypertrophic effects.

Structured PICO

P
Population
Preclinical model of Angiotensin II (Ang II)-induced cardiac hypertrophy
I
Intervention
Modulation of miR-300-3p and ACOX1 expression
C
Comparator
Control models
O
Outcome
Cardiac hypertrophy and ferroptosissurrogate

The study identifies the miR-300-3p/ACOX1/GPX4 axis as a novel regulatory pathway in cardiac hypertrophy and ferroptosis, offering potential new therapeutic targets.

Cite This Study

Li et al. (2026) studied Cardiac hypertrophy. miR-300-3p was evaluated on Ang II-induced cardiac hypertrophy and ferroptosis. DNMT1-regulated promoter hypomethylation enhances miR-300-3p expression, which suppresses ACOX1 and promotes ferroptosis in Ang II-induced cardiac hypertrophy.

synapsesocial.com/papers/6a2e48b0b1cc60ccdea8ca7chttps://doi.org/10.1016/j.bbrc.2026.154145
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