First Report of Storage Tuber Rot in Sweetpotato (Ipomoea batatas) Caused by Plenodomus destruens in Korea

HomePlant DiseaseVol. 103, No. 5First Report of Storage Tuber Rot in Sweetpotato (Ipomoea batatas) Caused by Plenodomus destruens in Korea PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Storage Tuber Rot in Sweetpotato (Ipomoea batatas) Caused by Plenodomus destruens in KoreaN. C. Paul, S.-S. Nam, W. Park, J.-W. Yang, and A. KachrooN. C. PaulBioenergy Crop Research Institute, National Institute of Crop Science, Rural Development Administration, Muan 58545, Republic of Korea; and , S.-S. NamBioenergy Crop Research Institute, National Institute of Crop Science, Rural Development Administration, Muan 58545, Republic of Korea; and , W. ParkBioenergy Crop Research Institute, National Institute of Crop Science, Rural Development Administration, Muan 58545, Republic of Korea; and , J.-W. Yang†Corresponding author: J.-W. Yang; E-mail: E-mail Address: hg369732@korea.krhttp://orcid.org/0000-0002-1547-8918Bioenergy Crop Research Institute, National Institute of Crop Science, Rural Development Administration, Muan 58545, Republic of Korea; and , and A. KachrooDepartment of Plant Pathology, University of Kentucky, Lexington, KY, U.S.A.AffiliationsAuthors and Affiliations N. C. Paul1 S.-S. Nam1 W. Park1 J.-W. Yang1 † A. Kachroo2 1Bioenergy Crop Research Institute, National Institute of Crop Science, Rural Development Administration, Muan 58545, Republic of Korea; and 2Department of Plant Pathology, University of Kentucky, Lexington, KY, U.S.A. Published Online:25 Feb 2019https://doi.org/10.1094/PDIS-10-18-1776-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Sweetpotato, Ipomoea batatas L., contains substantial levels of protein, carbohydrates, vitamins, and minerals and is popular in tropical and subtropical countries in Asia (China, Korea, and Japan), the Pacific Islands, Latin America, and Africa. Sweetpotato foot and storage tuber rot caused by the fungus Plenodomus destruens Harter are significant threats to the production of sweetpotato worldwide (Loebenstein and Thottappilly 2009). This fungus can cause disease on seedlings, mature plants, and storage roots. Decay or rot begins at the attached end, preliminarily as slight sunken lesions that become larger with time. Storage tuber rot caused by P. destruens was first observed in China in 2014 (Gai et al. 2016). Outbreaks of storage tuber rot disease were frequently observed on sweetpotato storage roots in Korea from July to August in 2015 to 2018, and it was important to confirm the cause of the disease. We collected samples from storehouses and surface sterilized with 1% NaOCl for 5 min, washed three times with sterilized distilled water, cut into small pieces, placed onto potato dextrose agar (PDA), and isolated. The selected fungi were stored at the Sweetpotato Research Laboratory, Bioenergy Crop Research Institute, Rural Development Administration, Muan, Korea (isolates SPL15025, SPL18007, and SPL18008). The representative isolate SPL15025 was cultured for 14 days on PDA and kept at 25°C with a 12-h light/dark photoperiod for sporulation. Different sized conidiomata observed and single-celled oblong to ellipsoid conidia with rounded ends were produced, measuring 6.4 to 10.6 × 1.9 to 3.9 μm (average 8.2 × 2.8 μm). The internal transcribed spacer (ITS) and translation elongation factor 1-α (EF-1α) genes were sequenced and analyzed to confirm species identity. The rDNA-ITS region was amplified with primers ITS5 and ITS4 (White et al. 1990), and EF-1α was amplified with primers EF 1 and EF 2 (O'Donnell 2000). These sequences were deposited in GenBank (accession nos. MH465671 for ITS and MH560611 for EF-1α). BLASTn search analysis showed 99 to 100% sequence similarity with P. destruens references (accession nos. JX421687/ITS and KP990656/EF-1α). Based on the conidial characteristics and DNA sequence analysis, the fungus was identified as P. destruens (Gai et al. 2016). Pathogenicity assays were conducted on two local sweetpotato varieties (Annobeni and Yonghwangmi) using a 5-mm P. destruens plug, with three replications, and a blank PDA plug served as a control. After 3 weeks of incubation in plastic containers at 25°C with high humidity, typical symptoms were detected. No symptoms were observed on the control samples, and P. destruens was reisolated from the artificially inoculated storage roots. To the best of our knowledge, this is the first report of storage tuber rot of sweetpotato caused by P. destruens in Korea.References:Gai, Y. et al. 2016. J. Gen. Plant Pathol. 82:181. https://doi.org/10.1007/s10327-016-0661-z Crossref, ISI, Google ScholarLoebenstein, G., and Thottappilly, G., eds. 2009. The Sweet Potato. Springer Science+Business Media, Berlin, Germany. Google ScholarO'Donnell, K. 2000. Mycologia 92:919. https://doi.org/10.2307/3761588 Crossref, ISI, Google ScholarWhite, T. J., et al. 1990. Page 315 in: PCR Protocols: A Guide to Methods and Applications. Academic Press, San Diego, CA. Crossref, Google ScholarFunding: This research was supported by the Basic Research Program funded by the Rural Development Administration, Republic of Korea (no. PJ01125502).DetailsFiguresLiterature CitedRelated Vol. 103, No. 5 May 2019SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionTomato leaves infected with Tomato chlorotic dwarf viroid (Olmedo-Velarde, Roy, Belanger, Watanabe, Hamasaki, Mavrodieva, Nakhla, and Melzer). Photo credit: M. J. Melzer. Spinach plants with downy mildew symptoms caused by Peronospora effusa (Kandel, Mou, Shishkoff, Shi, Subbarao, and Klosterman). Photo credit: S. L. Kandel. Metrics Article History Issue Date: 8 May 2019Published: 25 Feb 2019First Look: 19 Nov 2018Accepted: 15 Nov 2018 Pages: 1020-1020 Information© 2019 The American Phytopathological SocietyFundingRural Development AdministrationGrant/Award Number: PJ01125502Cited byGeneration and soil detection of nitrate-nonutilizing mutants of Diaporthe destruens causing foot rot of sweet potato9 February 2022 | Journal of General Plant Pathology, Vol. 88, No. 2Foot rot disease of sweet potato in Japan caused by Diaporthe destruens: first report, pathogenicity and taxonomy17 November 2021 | Journal of General Plant Pathology, Vol. 88, No. 1Management measures to reduce sweet potato foot rot caused by Diaporthe destruens in seed potatoes during storageJapanese Journal of Phytopathology, Vol. 87, No. 4High-Quality Genome Resource of Diaporthe destruens Causing Foot Rot Disease of Sweet PotatoLifei Huang, Xinxin Zhang, Yiling Yang, Hongda Zou, Boping Fang, and Weiming Liu26 October 2021 | Plant Disease, Vol. 105, No. 10Real-Time PCR Assay for the Diagnosis and Quantification of Co-infections by Diaporthe batatas and Diaporthe destruens in Sweet Potato22 June 2021 | Frontiers in Plant Science, Vol. 12