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June 7, 2013Circulation ResearchOpen Access

HCN4 Dynamically Marks the First Heart Field and Conduction System Precursors

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Population

Mouse models (HCN4CreERT2, -nuclear LacZ, and -H2BGFP mouse lines)

Design

Preclinical

Key result

HCN4 expression marked the first heart field at cardiac crescent stages and distinct cardiac conduction system precursors throughout embryonic development in mouse models.

Authors

XLXingqun LiangGWGang WangLLLizhu Lin

Discussion

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Overview

Facilitates HCN4 lineage tracing in mouse cardiac development; leaves open translation to human conduction disorders or biological pacemakers.

Structured PICO

P
Population
Mouse models (HCN4CreERT2, -nuclear LacZ, and -H2BGFP mouse lines)
E
Exposure
Lineage tracing and immunostaining using HCN4CreERT2, Isl1Cre, Nkx2.5Cre, and Tbx18Cre
O
Outcome
HCN4 expression and lineage tracing of the first heart field and cardiac conduction system precursorssurrogate

HCN4 is identified as a dynamic marker for the first heart field and cardiac conduction system precursors, facilitating lineage tracing and research into biological pacemakers.

Cite This Study

Liang et al. (2013) studied Cardiac conduction system development. HCN4 lineage tracing was evaluated on HCN4 expression and lineage tracing. HCN4 expression marked the first heart field at cardiac crescent stages and distinct cardiac conduction system precursors throughout embryonic development in mouse models.

synapsesocial.com/papers/6a320edca94b9b73a2cc05f7https://doi.org/10.1161/circresaha.113.301588
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