Peer-reviewed report characterizes antibodies for PPP2R5D, indicating best practices for researchers.
A peer-reviewed antibody characterization article corresponding to this Zenodo preprint is openly available at F1000Research: https://doi.org/10.12688/f1000research.145146.2 This report presents a guide to selecting high-quality commercial antibodies against Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform (PPP2R5D) by Western blot, immunoprecipitation, immunofluorescence and immunohistochemistry, using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. This study was funded in part by the Simons Foundation Autism Research Initiative (SFARI), an organization that funds innovative research to enhance the understanding, diagnosis and treatment of autism spectrum disorders.
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Ayoubi et al. (2023) studied this question.
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