Engineering protein interfaces yields ferritin disassembly and reassembly under benign experimental conditions

Ferritin nanocages are promising platforms for drug encapsulation. However, extreme conditions (pH ≤ 2) required for dissociation limit their application. Here, we engineered protein interfaces to yield ferritin nanocages which disassemble at pH 4.0 and reassemble at pH 7.5. During this process, bioactive molecules can be encapsulated within the protein cavity.