October 1, 1990AJP Heart and Circulatory Physiology
Dichloroacetate stimulation of glucose oxidation improves recovery of ischemic rat hearts
Key result
The addition of 1 mM dichloroacetate at reperfusion significantly improved the recovery of mechanical function in ischemic rat hearts to 73% of preischemic function compared to 37% in controls.
Why the study?
Does dichloroacetate improve mechanical recovery in isolated working rat hearts subjected to ischemia and reperfusion?
Population
Isolated working rat hearts subjected to a 30-min period of no flow ischemia, followed by a 30-min period of…
Comparison
1 mM dichloroacetate added to the perfusate at… vs Control hearts without dichloroacetate
Design
Preclinical
Follow-up
30 minutes of reperfusion
Authors
Discussion
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Implication
Does not support clinical use; leaves open whether dichloroacetate improves human ischemia-reperfusion recovery.
Key Points
- This study aims to investigate whether dichloroacetate can mitigate the inhibitory effects of fatty acids on glucose oxidation and enhance recovery in ischemic rat hearts.
- Isolated working rat hearts were perfused with glucose, palmitate, and insulin, subjected to 30 minutes of ischemia followed by reperfusion.
- Dichloroacetate was administered either at reperfusion or before ischemia to evaluate its effects on recovery and glucose oxidation rates.
- 14C-labeled glucose was used to measure glucose oxidation by collecting 14CO2 produced by the hearts.
- Control hearts recovered 37% of preischemic function after reperfusion, while addition of 1 mM dichloroacetate improved this to 73%.
- In nonischemic hearts, dichloroacetate significantly increased glucose oxidation rates from 298 to 1,223 nmol.g dry wt-1.min-1.
- Dichloroacetate was ineffective when administered before ischemia, leading to increased lactate accumulation during that period.
Structured PICO
Does dichloroacetate improve mechanical recovery in isolated working rat hearts subjected to ischemia and reperfusion?
P
Population
Isolated working rat hearts subjected to transient global ischemia and reperfusion.
I
Intervention
1 mM dichloroacetate added to the perfusate at reperfusion (or before the onset of ischemia)
C
Comparator
Control hearts without dichloroacetate
O
Outcome
Recovery of mechanical functionsurrogate
Main Result
Absolute Event Rate: 73% vs 37%
Dichloroacetate improves mechanical recovery of ischemic rat hearts when administered at reperfusion by stimulating glucose oxidation.
Cite This Study
McVeigh et al. (1990) studied Transient global ischemia. Dichloroacetate vs. Control was evaluated on Recovery of mechanical function (% of preischemic function). The addition of 1 mM dichloroacetate at reperfusion significantly improved the recovery of mechanical function in ischemic rat hearts to 73% of preischemic function compared to 37% in controls.