Key result
Moderate hypothermia (25°C) produced a significant but partial inhibition of sarcolemmal NHE activity in rat ventricular myocytes, without significantly affecting the inhibitory potency of cariporide.
Why the study?
Does moderate hypothermia affect sarcolemmal NHE activity and the inhibitory potency of cariporide in isolated adult rat ventricular myocytes?
Population
Isolated adult rat ventricular myocytes (n = 8 to 11 per group)
Comparison
Moderate hypothermia and cariporide vs Normothermic control group (37 degrees C)
Design
Preclinical
Authors
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May inform hypothermic cardioprotection strategies; leaves open translation to human myocytes or in vivo models.
Does moderate hypothermia affect sarcolemmal NHE activity and the inhibitory potency of cariporide in isolated adult rat ventricular myocytes?
Moderate hypothermia partially inhibits sarcolemmal NHE activity but does not alter the inhibitory potency of cariporide in rat ventricular myocytes.
Hoshino et al. (2001) studied this question. Moderate hypothermia (25 degrees C) and cariporide vs. Normothermic control (37 degrees C) was evaluated on Trans-sarcolemmal acid efflux rate (J(H)) and NHE-inhibitory potency of cariporide (IC50). Moderate hypothermia (25°C) produced a significant but partial inhibition of sarcolemmal NHE activity in rat ventricular myocytes, without significantly affecting the inhibitory potency of cariporide.