Functional Interaction between the p160 Coactivator Proteins and the Transcriptional Enhancer Factor Family of Transcription Factors

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Key Points

To explore the interaction between p160 coactivators and TEF transcription factors, and their function in transcriptional activation.
Conducted yeast two-hybrid screening using the bHLH-PAS domain of SRC1 as bait.
Performed transient transfection experiments to assess transcriptional activity from TEF response elements.
Utilized COS-1 and HeLa cells for conducting necessary assays and experiments.
SRC1 coactivator enhances transcriptional activation via TEF elements (p<0.01).
All members of the p160 family (SRC1, TIF2, RAC3) potentiate TEF-mediated transcription (p<0.05).
TEF-4 demonstrated specific binding to the bHLH-PAS domain of SRC1 (β-galactosidase activity >5x control).

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Shows coactivators enhance transcription in relation to TEF factors, indicating their critical role.

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Belandia et al. (2000) studied this question.

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