Pulmonary ionocytes are rare epithelial cell that bear the highest amount of mRNA for CFTR and whose function is necessary to regulate airway surface liquid homeostasis. Lineage induction is regulated by the transcription factor Foxi1, but the temporal and spatial localization of Foxi1 + cells during airway development and the impact of CFTR mutations remain poorly understood. Here, we used immunofluorescence and RNAscope to detect Foxi1 + cells throughout postnatal airway development in male and female mice, from neonatal stages to one year of age, including CftrΔF508 mutant animals in adult stage. Foxi1 + cells were observed in higher density in the laryngeal subglottis region, decreasing towards the distal trachea, and principally located in the surface epithelium covering intercartilage zones. The Foxi1 + cells were observed at postnatal day 7.5 and their appearance correlated to the development of submucosal glands. The distribution of these cells was not altered in the Cftr ΔF508/ΔF508 mutant mouse. These findings demonstrate that pulmonary ionocytes display a highly regionalized distribution pattern in the mouse airway and emerge during a defined postnatal developmental window associated with submucosal gland maturation. Their localization near submucosal gland openings in the mouse trachea and co-appearance during development suggests a potential role in regulating submucosal gland secretion and airway epithelial fluid homeostasis.
Villanueva et al. (Tue,) studied this question.