Why the study?
Increased sensitivity of high-resolution flow cytometers introduces artifacts like antibody aggregates, which can interfere with the detection of extracellular vesicles.
Filtering protein labels reduces antibody aggregates in high-resolution flow cytometry, but appropriate controls remain necessary for extracellular vesicle studies.
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Antibody aggregates may confound rare EV detection by hFCM; leaves open optimized pre-treatment protocols for reliable vesicle phenotyping.
Rasmussen et al. (2021) studied this question.
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