Key points are not available for this paper at this time.
2D cell culture models on EV production and analysis. In this review, we examine recent studies that compare the impacts of 3D and 2D cell culture models on EV yield, composition, and functionality. We categorize 3D models into subtypes, including spheroids, hydrogels, rigid scaffolds, and bioreactors. Details of each model's impact on EVs compared to 2D cell culture are presented. Furthermore, we discuss the advantages and limitations of these 3D models as identified in individual studies, offering insights to guide future research directions in this evolving field.
Cook et al. (Wed,) studied this question.