Comparison of the effects of pure vitamin E and combined conservative therapy for precancerous cervical lesions cultivated on a microfluidic chip

Objectives We aimed to examine the effect of vitamin E on preinvasive cervical squamous epithelium lesions on a microfluidic chip model. Methods The research was conducted on 60 women, previously determined for a biopsy of the cervix due to an abnormal screening cytological test. The control group consisted of patients who were not treated with vaginal tablets Ialuna ® or pure vitamin E. Samples were cultivated on a microfluidic chip, treated with Ialuna ® vaginal tablets containing vitamin E combined with other ingredients or pure vitamin E, and then relative gene expression of BAX and BCL2 genes was measured. Results In tissues from women with chronic cervicitis, treatment with Ialuna ® vaginal tablets induced a significant increase in BAX expression and the BAX/BCL2 ratio, while no significant changes were observed in BCL2 expression. In LSIL tissues, Ialuna ® treatment resulted in a significant decrease in BCL2 expression, accompanied by increased BAX expression and an elevated BAX/BCL2 ratio. Conversely, pure vitamin E treatment did not produce significant changes in apoptotic markers in either group. Conclusions This study has shown that conservative therapy is an important part of the treatment of cervical precancerous lesions and can potentially lead to diminishing radical interventions.