Cosmetic Anti-Aging Potential of the Traditional Thai Longevity Formula Mai-Kae-Den-Klong: Mechanistic Insights from Enzyme-Based Bioassays and In Silico Analysis
Randomized trial shows promising anti-aging effects of Mai-Kae-Den-Klong in cosmetic applications, suggesting natural alternatives can enhance skin health.
Key Points
This study aims to explore the anti-aging potential of the traditional Thai herbal formula Mai-Kae-Den-Klong (MKDK) using enzyme bioassays and in silico analysis.
Investigated antioxidative and anti-aging properties of MKDK using enzyme-based bioassays and in silico methods.
Conducted LC-MS analysis for phytochemical profiling of MKDK extract.
Assessed inhibitory activities against skin-aging enzymes: tyrosinase, collagenase, elastase, and hyaluronidase.
MKDK extract showed strong antioxidant activity with IC50 values of 17.23 µg/mL (DPPH) and 11.87 µg/mL (ABTS).
Exhibited inhibitory effects with IC50 values: tyrosinase 41.25 µg/mL, elastase 49.51 µg/mL, collagenase 61.54 µg/mL, and hyaluronidase 63.74 µg/mL.
Molecular docking revealed favorable binding of key metabolites to skin-aging-related enzymes.