displays a spectrum of phase behaviors: independent phase separation with mucin, partial co-condensation with γ-globulin, and robust heterotypic LLPS with bovine serum albumin (BSA), lysozyme, and β-lactoglobulin. Confocal laser scanning microscopy (CLSM) imaging and FRAP analysis reveal that protein co-condensation can modulate condensate diffusivity, with shared compartments dampening dynamics and distinct ones enhancing them. Our findings highlight atomically precise nanoclusters as a powerful alternative luminescent analogue for dissecting biomolecular LLPS and elucidating nanobio interactions.
Mukhopadhyay et al. (Thu,) studied this question.