Dirhamnolipids from Pseudomonas aeruginosa PAO1 Protect Hairless Mouse Skin from UVB-Induced Inflammation and Oxidative Stress

High Resolution Image Download MS PowerPoint Slide Frequent and excessive skin exposure to ultraviolet radiation induces oxidative stress and inflammation, raising skin diseases. Therapeutic strategies targeting both oxidative stress and inflammation may reduce skin disorders. Rhamnolipids are highly effective surface-active glycolipid biosurfactants with enormous market potential. Although these green amphiphilic molecules exhibit remarkable biomedical potential, including antimicrobial activity, wound healing properties, and analgesic effect on inflammatory pain, their therapeutic potential in UVB induced skin inflammation has not yet been investigated. A glycolipid product enriched in Dirhamnolipids (95.7%) was obtained, followed by single-step purification that provided a highly pure fraction containing 99.5% total rhamnolipids and 98.9% Dirhamnolipids (purified Di-RL). Treatment with Di-RL reduced inflammation and oxidative stress in UVB-irradiated hairless mouse skin. At a dose of 3 mg/kg, purified Di-RL reduced edema, epidermal thickening, leukocyte infiltration, myeloperoxidase activity, mast cell counts, antioxidant capacity depletion, lipid peroxidation, superoxide anion levels, and TNFα production. Collectively, these results underscore the therapeutic potential of Di-RL in the management of UVB-induced skin inflammation and oxidative stress.