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June 21, 2026Molecular Biology ReportsOpen Access

CRISPR/Cas9-mediated miR-21 editing in high-grade urothelial carcinoma cells and its biological effects

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Authors

MLM. LeeJCJuliana Alves de CamargoGBGiovana Caetano Vilas Boas

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Overview

Randomized trial investigates CRISPR/Cas9 effects on tumor suppressors in high-grade urothelial carcinoma, suggesting potential therapeutic avenues.

Key Points

  • To examine the effects of CRISPR/Cas9-mediated editing of miR-21 on urothelial carcinoma cells.
  • Used CRISPR/Cas9 delivered as a ribonucleoprotein complex
  • Assessed editing efficiency with RT-qPCR
  • Evaluated functional effects using gene expression, cell migration, and Matrigel invasion assays.
  • Reduced miR-21 expression observed (p = 0.0563)
  • Significant increase in MASPIN (p < 0.0001) and PDCD4 (p = 0.0239) expression
  • Significant reduction in migratory capacity after 48 hours (p = 0.0334).

Cite This Study

Lee et al. (2026) studied this question.

synapsesocial.com/papers/6a3781db24f042ddf4c5b7e4https://doi.org/10.1007/s11033-026-12129-7
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