Key result
A direct enzyme immunoassay using horseradish peroxidase-labeled monoclonal antibody UM 21.1 rapidly detected EMCV multiplication in L-cells and allowed rapid titration of neutralizing antibodies.
Why the study?
Does an enzyme immunoassay using horseradish peroxidase-labeled monoclonal antibodies allow for rapid detection of EMCV, neutralizing antibodies, and interferon in cell culture?
Population
L-cell monolayers infected with Encephalomyocarditis virus (EMCV)
Comparison
Direct enzyme immunoassay using EMCV-specific… vs Conventional plaque reduction test
Design
Preclinical
Authors
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Enables faster in vitro EMCV assays; leaves open translation to human diagnostics or clinical use.
Does an enzyme immunoassay using horseradish peroxidase-labeled monoclonal antibodies allow for rapid detection of EMCV, neutralizing antibodies, and interferon in cell culture?
A direct enzyme immunoassay using labeled monoclonal antibodies provides a rapid and reliable method for detecting EMCV and titrating interferon and neutralizing antibodies in cell culture.
Vlaspolder et al. (1988) studied Encephalomyocarditis virus (EMCV) infection (in vitro). Direct enzyme immunoassay using EMCV-specific monoclonal antibody UM 21.1 vs. Conventional plaque reduction test was evaluated on Detection of virus multiplication, interferon, and neutralizing antibodies. A direct enzyme immunoassay using horseradish peroxidase-labeled monoclonal antibody UM 21.1 rapidly detected EMCV multiplication in L-cells and allowed rapid titration of neutralizing antibodies.
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