The extracellular polysaccharide (EPS) released by a freshwater desmid, Staurastrum orbiculare (Zygnematophyceae), was characterized and their degradation by heterotrophic microbial populations was also evaluated. The EPS was purified by anion exchange column chromatography, the monosaccharide composition was determined by gas chromatography and the linkages of the monosaccharides by gas chromatographic analysis coupled with electron impact mass spectrometry. The EPS was mainly eluted in one weak acid fraction, although a smaller strong acid fraction was also eluted. The two fractions had similar composition and both of them were mainly composed of fucose, glucuronic acid, galactose, galacturonic acid, xylose, glucose, rhamnose and N-acetyl glucosamine. We monitored the entire EPS degradation, and also their components separately, during 35 days using pulse amperometric detection in a high-performance liquid chromatography. Fucose, galactose and especially glucose showed a faster degradation patt...
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Giroldo et al. (2005) studied this question.
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