Key result
Using a modified APEX2 approach with SILAC, emerin, TMEM43, and ELYS were identified as potential interaction partners of VAPB at the inner nuclear membrane and nuclear pore complex.
A modified APEX2 approach identified emerin, TMEM43, and ELYS as novel interaction partners of VAPB at the inner nuclear membrane.
No immediate clinical implications; leaves open the functional roles of these VAPB partners pending validation.
Vesicle-associated membrane protein-associated protein B (VAPB) is a tail-anchored protein that is present at several contact sites of the endoplasmic reticulum (ER). We now show by immunoelectron microscopy that VAPB also localizes to the inner nuclear membrane (INM). Using a modified enhanced ascorbate peroxidase 2 (APEX2) approach with rapamycin-dependent targeting of the peroxidase to a protein of interest, we searched for proteins that are in close proximity to VAPB, particularly at the INM. In combination with stable isotope labeling with amino acids in cell culture (SILAC), we confirmed many well-known interaction partners at the level of the ER with a clear distinction between specific and nonspecific hits. Furthermore, we identified emerin, TMEM43, and ELYS as potential interaction partners of VAPB at the INM and the nuclear pore complex, respectively.
No takes yet. Share an insight, caveat, or question.
James et al. (2019) studied this question. Rapamycin-dependent targeting of APEX2 was evaluated on Identification of binding partners of VAPB at the inner nuclear membrane. Using a modified APEX2 approach with SILAC, emerin, TMEM43, and ELYS were identified as potential interaction partners of VAPB at the inner nuclear membrane and nuclear pore complex.
Synapse has enriched 5 related papers. Consider them for comparative context: