PCPE-1, a brown adipose tissue-derived cytokine, promotes obesity-induced liver fibrosis

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  Figure 1. Exploration of BAT-derived adipokine in obesity. (A) Results of DNA microarray datasets analyzing brown adipose tissue (BAT) from adipose tissue-specific Vegfa knockout mice (Adipo-Vegfa KO) (GSE221854) or high-fat diet (HFD) fed wild-type mice (diet-induced obesity (DIO)) (GSE28440). (B) C57BL/6NCrSlc mice were fed with a normal chow (NC) or a high-fat diet (HFD) from 4 weeks of age and studied at 34–38 weeks of age. Hematoxylin-eosin (HE) staining of BAT from indicated mice. Scale bar = 100 μm. (C) Copy number for the transcript Pcolce in BAT, liver, epididymal white adipose tissue (eWAT) and skeletal muscle (SM) (quadriceps) of the indicated mice (all n = 4). (D) Immunofluorescent staining for procollagen C-endopeptidase enhancer-1 (PCPE-1) in BAT of the indicated mice. Scale bar = 50 μm. The right panel indicates the quantification of the PCPE-1 positive area in BAT (n = 4, 4). (E) Western blot analysis of PCPE-1 expression in BAT from the indicated mice. The right panel indicates the quantification of PCPE-1 relative to Actin (n = 5, 6). (F) ELISA for PCPE-1 in plasma from the indicated mice (n = 7, 8). (G) HE staining of the liver from the indicated mice. Scale bar = 100…

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  Figure EV1. Related to Fig. 1. C57BL/6NCrSlc wild-type (WT) mice were fed with a normal chow (NC) or a high-fat diet (HFD) from 4 weeks of age and studied at 38–40 weeks of age. (A, B) Body weight (BW) (A) (n = 4, 6) or brown adipose tissue (BAT) weight (B) (n = 4, 5) of indicated mice. (C) Reference Expression Dataset (RefEx) showing transcript Pcolce in systemic organs. (D–J) Gene Expression Omnibus studies showing transcript Pcolce in eWAT and BAT (D) (n = 3, 3 in GSE8044). The transcript was also tested under a HFD fed condition in mice in BAT (E) (n = 3, 3 in GSE64718), eWAT (n = 4, 4), liver (n = 4, 4), skeletal muscle (SM) (F) (n = 3, 4 in GSE123394), bone (G) (n = 3, 4 in GSE194075), adrenal gland (H) (n = 4, 4 in GSE216327), skin (I) (n = 5, 5 in GSE96932) and in heart (J) (n = 4, 4 in GSE171710). (K–M) Tabula Muris Senis testing transcript Pcolce in systemic organs (K), or in cells in the heart (L) or BAT (M). (N–P) Results from quantitative PCR (qPCR) showing transcript Pcolce in primary cardiac fibroblasts and primary brown adipocytes (N) (n = 6, 6), primary hepatocytes and primary brown adipocytes (O) (n = 5, 5), and hepatocyte (AML12) or brown adipocyte cell lines (P) (n = 11,…

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  Figure 2. BATokine PCPE-1 promotes liver fibrosis in MASH. Littermate control (Con) or BAT-specific Pcolce knockout mice (BAT Pcolce KO) were fed a high-fat diet (HFD) from 4 weeks of age and studied at 42–43 weeks of age. (A) Quantitative PCR (qPCR) results showing transcript Pcolce in BAT from indicated mice (n = 6, 6). (B) Western blot analysis for procollagen C-endopeptidase enhancer-1 (PCPE-1) in BAT from indicated mice. The quantification results for this panel see Fig. EV2C. (C) ELISA for plasma PCPE-1 (n = 6, 6) in the indicated mice aged 42–43 weeks. (D) Western blot analysis for PCPE-1 in liver from indicated mice. The right panel indicates the quantification (n = 3,5). (E) Picrosirius red staining of liver from the indicated mice. Scale bar = 500 μm. For the quantification results see Fig. EV2G. (F–H) ELISA for liver collagen type I (F) (n = 5, 5), liver triglyceride (TG) (G) (n = 35, 27), and plasma alanine transaminase (ALT) (H) (n = 8, 8) in the indicated mice aged 42–43 weeks. (I) Results from qPCR showing transcripts Col1a1 (n = 6, 6), Ccn2(Ctgf) (n = 5, 6), Tgfb1 (n = 5, 6), Ccl2 (n = 5, 6), and Tnf (n = 6, 6) in liver from indicated mice. (J) Experimental design of the…

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  Figure EV2. Related to Fig. 2. Brown adipose tissue (BAT)-specific Pcolce knockout (KO) mice (UCP1-Cre+/-; Pcolceflox/flox; BAT Pcolce KO) or littermate control mice (UCP1-Cre-/-; Pcolceflox/flox; Con) were fed a high-fat diet (HFD) from 4 weeks of age and studied at 42–43 weeks of age. (A) Design of the BAT Pcolce KO mice. (B) Results from quantitative PCR (qPCR) showing transcript Pcolce in the liver (n = 5, 6), epididymal white adipose tissue (eWAT) (n = 6, 6), heart (n = 5, 6), and skeletal muscle (SM) (quadriceps) (n = 6, 6) from indicated mice fed a HFD. (C) The quantification of western blot analysis for PCPE-1 relative to Actin in BAT (n = 3, 5) related to Fig. 2B. (D, E) Body weight (BW) (D) (n = 16, 7), or BAT weight (E) (n = 16, 7) of indicated mice. (F) HE staining of the BAT from the indicated mice. Scale bar = 50 μm. (G) Relative fibrotic area in liver in the indicated mice, related to Fig. 2E (n = 5, 6). (H) ELISA for liver collagen type I (Collagen1) in the indicated mice (n = 5, 5). (I) Results from quantitative PCR (qPCR) showing transcript Pcolce in heart (n = 4, 5), liver (n = 5, 5) and kidney (n = 5, 5) in the indicated mice. (J) Relative fibrotic area of the liver in…

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  Figure 3. Models with systemic PCPE-1 inhibition ameliorate liver fibrosis. Littermate control (Con) or systemic Pcolce KO mice were fed a normal chow (NC) or a high-fat diet (HFD) from 4 weeks of age and studied at 38–40 weeks of age. (A) Quantitative PCR (qPCR) results showing transcript Pcolce in BAT from indicated mice (n = 6, 6, 6, 6). (B–D) Western blot analysis for PCPE-1 in BAT (B) or liver (D), and ELISA showing plasma PCPE-1 (C) (n = 6, 6, 6, 6) from indicated mice. For the quantification of these Western blot results see Fig. EV3B or Fig. EV3E. (E) Picrosirius red staining of liver from the indicated mice. Scale bar = 500 μm. For the quantification results see Fig. EV3F. (F–H) ELISA for liver collagen type I (F) (n = 5, 5, 5, 5), liver triglyceride (TG) (G) (n = 8, 8, 8, 8), and plasma alanine transaminase (ALT) (H) (n = 6, 8, 8, 8) (G) in the indicated mice. (I) Results from qPCR showing transcript Col1a1, Ccn2(Ctgf), Tgfb1, Ccl2, and Tnf in liver from indicated mice (n = 6, 6, 6, 6). (J) Experimental design of a vaccination therapy targeting PCPE-1. C57BL/6NCrSlc mice were fed a HFD from 4 weeks of age. These mice were injected with a PCPE-1 vaccine at 12, 16, and 24 weeks of…

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  Figure EV3. Related to Fig. 3. Systemic Pcolce knockout (Pcolce KO) or littermate control (Con) mice were maintained on a HFD from 4 weeks of age, and tissues were harvested and analyzed at 38–44 weeks of age. (A) Results from quantitative PCR (qPCR) showing transcript Pcolce in the liver, epididymal white adipose tissue (eWAT), heart, and skeletal muscle (SM) (quadriceps) from the indicated mice (heart: n = 5, 6, 6, 6, all other organs: n = 6, 6, 6, 6). (B) The quantification of western blot analysis for PCPE-1 relative to GAPDH in BAT related to Fig. 3B (n = 5, 6, 6, 6). (C, D) Body weight (BW) (n = 10, 9, 7, 11) (C), or BAT weight (n = 10, 9, 7, 11) (D) of indicated mice. (E) The quantification of western blot analysis for PCPE-1 relative to Actin in liver related to Fig. 3D (n = 5, 5, 5, 5). (F) Relative fibrotic area in the liver of indicated mice, related to Fig. 3E (n = 5, 5, 4, 8). (G) HE staining of the BAT from the indicated mice. Scale bar = 50 μm. (H) CT scan analyzing the abdominal area of indicated mice aged 29 weeks of age. (I) Volume of visceral or subcutaneous fat in the subdiaphragm abdominal area analyzed with a CT scan (n = 6, 4, 6, 6). (J) Glucose tolerance test (GTT) (n…

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  Figure 4. ER stress pathway increases PCPE-1 in brown adipocytes. (A) Western blot analysis for phospho c-Fos (p-c-Fos), c-Fos, phospho c-Jun (p-c-Jun), and c-Jun in brown adipose tissue (BAT) from C57BL/6NCrSlc wild-type (WT) mice fed a normal chow (NC) or a high-fat diet (HFD) (aged 32–38 weeks). The right panel indicates the quantification of p-c-Fos, c-Fos, p-c-Jun, and c-Jun relative to GAPDH (n = 4, 4). (B) Western blot analysis for c-Fos and c-Jun in BAT injected with adeno-associated virus (AAV) encoding c-Fos (AAV-c-Fos) co-injected with AAV-c-Jun (AAV-c-Fos+c-Jun) into this tissue. AAV study was done in 12-week-old WT mice maintained on a normal chow diet 7 days after the injection. The right panel indicates the quantification of c-Fos and c-Jun relative to Actin (n = 4, 4). (C, D) Results from quantitative PCR (qPCR) showing transcript Pcolce (n = 4, 4) (C) and western blot analysis for PCPE-1 (D) in BAT from indicated mice. The right panel in (D) indicates the quantification of PCPE-1 relative to GAPDH (n = 4, 4). (E, F) Results from qPCR showing transcript Pcolce (n = 6, 6) (E) and western blot analysis for PCPE-1 (F) in differentiated brown adipocytes introduced with mock or…

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  Figure EV4. Related to Fig. 4. (A) Results of in silico analysis (DBTSS [http://dbtss.hgc.jp] and JASPAR [http://jaspar.binf.ku.dk]) predicting c-Fos and c-Jun may bind to putative promotor region of Pcolce. (B) Western blot analysis for phospho-JNK1/2 (p-JNK1/2) and JNK1/2 in fully differentiated brown adipocytes administrated with or without palmitic acid (Palm, 100 μM). The right panel indicates the quantification of p-JNK1/2 (n = 6, 6) relative to GAPDH. (C–E) Western blot analysis for p-c-Fos, c-Fos, p-c-Jun, and c-Jun in fully differentiated brown adipocytes administrated with PBS, palmitic acid (Palm; 100 μM for 6 h) with or without an IRE1 inhibitor (STF-083010, 30 μM, pre-treated 1 h) (C) or a JNK inhibitor (SP600125, 2 μM, pre-treated 1 h) (D, E). The right panels indicate the quantification of p-c-Fos and p-c-Jun relative to GAPDH or Actin (C: n = 6, 6, 6 for p-c-Fos, n = 11, 12, 12 for p-c-Jun) (D: n = 4, 6, 6) (E: n = 5,6,6). (F–I) Results from quantitative PCR (qPCR) showing transcript Pcolce in the differentiated brown adipocytes administrated with Palm (E) (n = 6, 6), BRE (Brefeldin A) (G) (n = 4, 4), Tunicamycin (H) (n = 6, 8) or Thapsigargin (I) (n = 4, 4). Data in Fig.…