Key result
Recombinant foot-and-mouth disease viruses expressing HSV or FLAG epitopes stably maintained the foreign tags and induced tag-specific antibodies in mice, demonstrating potential as a viral vector.
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FMDV can be effectively used as a viral vector for the delivery of foreign tags by introducing epitopes into the 3A nonstructural protein.
Foot-and-mouth disease virus (FMDV) is an aphthovirus that belongs to the Picornaviridae family and causes one of the most important animal diseases worldwide. The capacity of other picornaviruses to express foreign antigens has been extensively reported, however, little is known about FMDV. To explore the potential of FMDV as a viral vector, an 11-amino-acid (aa) HSV epitope and an 8 aa FLAG epitope were introduced into the C-terminal different regions of 3A protein of FMDV full-length infectious cDNA clone. Recombinant viruses expressing the HSV or FLAG epitope were successfully rescued after transfection of both modified constructs. Immunofluorescence assay, Western blot and sequence analysis showed that the recombinant viruses stably maintained the foreign epitopes even after 11 serial passages in BHK-21 cells. The 3A-tagged viruses shared similar plaque phenotypes and replication kinetics to those of the parental virus. In addition, mice experimentally infected with the epitope-tagged viruses could induce tag-specific antibodies. Our results demonstrate that FMDV can be used effectively as a viral vector for the delivery of foreign tags.
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Li et al. (2012) studied Foot-and-mouth disease virus (FMDV). Recombinant FMDV expressing HSV or FLAG epitopes vs. Parental virus was evaluated on Expression and stability of foreign epitopes, plaque phenotypes, replication kinetics, and induction of tag-specific antibodies. Recombinant foot-and-mouth disease viruses expressing HSV or FLAG epitopes stably maintained the foreign tags and induced tag-specific antibodies in mice, demonstrating potential as a viral vector.
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