Real Time Intraoperative Confocal Laser Microscopy-Guided Surgery

In Brief Objective: To assess the potential utility of in vivo histologic surface and subsurface imaging in real-time using the Optiscan confocal laser microscope to detect diseased tissue at the time of surgery. Summary Background Data: The goal of surgical treatment of diseases such as cancer is complete microscopic resection of diseased tissue; however, current methods for the assessment of extent of disease at the time of surgery are inadequate. Methods: We assessed the potential of the Optiscan confocal laser microscope, a new device developed for real-time in vivo histologic surface and subsurface imaging during surgery. Results: Intravenous Fluorescein Sodium contrast enabled visualization of cellular and architectural morphology of intra-abdominal organs with magnification equivalent to light microscopy and enabled differentiation between normal organs and disease. Conclusions: Real time intraoperative confocal microscopy has significant potential application in detecting disease, and influencing decision-making at the time of surgery. The Optiscan confocal laser microscope detected diseased tissue at the time of surgery by providing in vivo histologic surface and subsurface images equivalent to light microscopy. Intraoperative confocal microscopy has significant potential application in detecting disease and influencing decision-making at the time of surgery.