Abstract Self-feeding systems have been used to study aquatic animals’ behavior, suggesting initial studies for practical diet design. This study aimed to identify gaps in knowledge of the behavior, feeding rhythms, and target protein intake of the Amazon River prawn ( Macrobrachium amazonicum ) to inform the design of new diets. This study included two trials. In trial 1, under a 12:12 h LD photoperiod, 40 prawns (mean ± SD = 2.18 ± 0.3 g) were divided into four experimental units. A self-feeding system connected by an infrared sensor was prepared. Prawns were allowed to trigger sensors connected to feeders to assess a possible daily feeding rhythm for 52 days. During trial 2, 80 prawns (1.55 ± 0.19 g) were placed inside eight aquariums. The effect of two dietary protein levels (20%P or 40%P) was evaluated on behavioral protein intake target using self-feeding, as well as growth and body composition. The results of trial 1 showed that Amazon River prawn exhibited predominantly nocturnal behavior using self-feeding systems, with higher food intake during this period ( p 0.05). These findings can help to understand feed schedules and nutritional approaches, which are important for new aquaculture species.
Queiroz-Anjos et al. (Mon,) studied this question.