Third-generation sequencing data of Rhizoctonia solani XEMS25-1

段宇航(yuanhang duan)

doi:10.26036/cnp0006193

June 30, 2026Open Access

Third-generation sequencing data of Rhizoctonia solani XEMS25-1

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Key Points

The study aims to analyze the genome of Rhizoctonia solani AG-3 strain XEMS25-1 using advanced sequencing techniques.
DNA was extracted from a single disease spot of Rhizoctonia solani AG-3 strain XEMS25-1.
Genome sequencing was performed using Pacific Biosciences Sequel II and Illumina NovaSeq PE150.
De novo assembly of sequencing data estimated a genome size of 21 Gb.
The combined sequencing data resulted in a genome assembly size of approximately 21 Gb.
Data integration from two platforms enhanced the genome assembly accuracy.

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Implication

Third-generation sequencing of Rhizoctonia solani XEMS25-1 reveals a 21 Gb genome size, suggesting significant genetic complexity.

Abstract

Rhizoctonia solani AG-3 strain XEMS25-1 (here referred as R.S. 32) was obtained from a single disease spot, its DNA was sequenced using Pacific Biosciences Sequel II (PacBio) and Illumina NovaSeq PE150 (Nova). Data from both sequencing platforms were combined and the de novo assembling estimated a 21 Gb genome size. Data from both sequencing platforms were combined and the de novo assembling estimated a 21 Gb genome size.

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段宇航(yuanhang duan) (2026) studied this question.

synapsesocial.com/papers/6a44ae605cd2549c8bc43960 https://doi.org/10.26036/cnp0006193

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Third-generation sequencing data of Rhizoctonia solani XEMS25-1

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Third-generation sequencing data of Rhizoctonia solani XEMS25-1

Key Points

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Abstract

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