Multi-omics analysis identifies altered CD8⁺ T-cell differentiation in transplant-associated cancers, suggesting implications for immune dysfunction.
Key Points
This research aims to understand how chronic immunosuppression impacts CD8⁺ T-cell differentiation in transplant-associated cutaneous squamous cell carcinoma.
Integrated single-cell transcriptomic and T-cell receptor repertoire analyses of TSCC and immunocompetent SCC.
Evaluated EOMES association with antitumor CD8⁺ T-cell differentiation using an independent SCC cohort.
Conducted spatial transcriptomic analysis to assess tumor microenvironment interactions.
Accumulation of precursor effector-memory CD8⁺ T cells in TSCC with reduced cytotoxic function and clonal expansion.
Predictive modeling identified EOMES as a crucial regulator of cytotoxic gene expression and differentiation.
CXCL10-CXCR3 signaling was found to be a dominant pathway affecting Pre-Tem cells and linked to impaired differentiation.