Publisher Correction: Subcellular visualization and quantification of cyanotoxin synthesis in cyanobacteria reveals distinct compartmentation

The original Figs. 1 and 4 and accompanying legends appear below. Fig. 1 Full size image Combining immunofluorescence (IF) labeling of MCs with chemoselective visualization of MCs in cells of M. aeruginosa strain Hofbauer ( A – D ). For this purpose, a double-labeling protocol for chemoselective labeling of clickable MCs (yellow fluorophore, click-A555-azide/alkyne) and the subsequent immunolabeling of MCs (green fluorophore, IF-sAB-A488) was established. M. aeruginosa cells were grown in the presence of non-AA, either Phe-Az, or Prop-Lys, or Prop-Tyr, to enable a click-chemistry reaction between clickable MC and A555-azide/alkyne. Control cells were grown in the absence of non-AA but treated identically. For immunofluorescence a monoclonal primary antibody (binding to the Adda side chain of the MC molecule) coupled to sAB-488 was used. The specificity of the secondary antibody sAB-488 was tested in absence of primary antibody (IF/C). Natural autofluorescence (AF) is observed in red. ( E – G ) The mean ± SE colocalization coefficients (Object Pearson and Object Spearman) were calculated to quantify the spatial correlation between different emission pairs: green channel (G) vs. red channel (R), i.e., IF-sAB-A488 vs. AF; yellow channel (Y) vs. R, i.e., click-A555 vs. AF; G/Y, i.e., IF-sAB-A488 vs. click-A555. For quantitative comparison of signal intensity between G and Y for both MC and another protein (RbcL, Rubisco large subunit) see Extended Data Fig. 1 .