⌘+K

Pulse Explore Communities Researchers Journals

Home Communities Explore

July 1, 2026European Journal of Immunology

Fast Generation of F(Ab’) ₂ Fragments From Human IgG Using Fc‐Fused IgG‐Degrading Enzyme

Emna Hannachi Robin V. Lacombe Amélia Trecco Victoria Daventure Sandrine Delignat Jordan D. Dimitrov Claire Deligne Sébastien Lacroix‐Desmazes

View at Publisher

Ask AI

Bookmark

Share

Key Points

The aim is to efficiently generate Fab fragments from human IgG while avoiding enzyme contamination.
Utilized Fc-fused IgG-degrading enzyme for fragment generation.
Collected fragments by centrifugation in original buffer.
Successfully generated Fab fragments with minimal contamination.
Fragments maintained functionality and purity levels throughout the process.

Implication

Demonstrates efficient fragment collection in IgG without contamination.

Abstract

fragments or Fc-free proteins are collected by centrifugation in the original buffer without IdeS contamination.

Discussion

No takes yet. Share an insight, caveat, or question.

Cite This Study

Hannachi et al. (2026) studied this question.

synapsesocial.com/papers/6a48a83589561a0c2d78ef94 https://doi.org/10.1002/eji.70235

View at Publisher

Ask AI

Bookmark

Share

Keep reading

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

1One-step cleavage and purification: a bifunctional IdeS-Protein A fusion protein for on-column generation of antibody fragmentsOriginal Research · 2026
2ABDEG mutations in engineered IgG1 Fc fragments balance FcRn engagement and molecular stability to maximize IgG degradationOriginal Research · 2026
3Protein G affinity chromatography is an underrated but very potent purification method for a broad range of species-independent and tag-less Fab-fragments2024 · 9 citations
4Evaluation of the impact of antibody fragments on aggregation of intact molecules via size exclusion chromatography coupled with native mass spectrometry2024 · 8 citations
5Aptamers Generated by Multi-Strategy Selection Target FcγRI and Block IgG Fc-FcγRI Interaction2025 · 2 citations

More related papers

Fab–Fc and Fab–Fab interactions of variable strength and valency contribute to the high concentration viscosity of IgG <sub>1</sub> antibodies2026
Novel human IgG1 and IgG4 Fc-engineered antibodies with completely abolished immune effector functions2016
Rapid Antibody Fragment Production and Binding Analysis Using Cell-Free Protein Synthesis Combined with Fluorescence Correlation Spectroscopy2025

More from European Journal of Immunology

Cross‐Neutralizing and Broadly Neutralizing Human Antibodies Against Nonenveloped VirusesThu,
PGE2 Enhances Human ILC3 Function but Constrains ILC2‐to‐ILC3 PlasticityThu,