Transcriptome sequencing of the mouse colonic anastomosis site

Transcriptome sequencing was performed on colonic anastomotic tissues collected from mice in this study. All experimental animals were divided into two groups: the solvent control group and the intervention group with Streptococcus anginosus colonization. The intervention group was treated with Streptococcus anginosus to induce stable bacterial colonization at the anastomotic site, while the control group received only corresponding solvent treatment without bacterial intervention. At the preset time points after surgery, local tissues around the colorectal anastomosis were isolated, and total RNA was extracted for high-throughput transcriptome sequencing. The obtained transcriptomic data were used to screen differentially expressed genes, analyze altered signaling pathways, and explore the molecular mechanisms by which Streptococcus anginosus colonization affects the repair and healing process of colorectal anastomosis in mice