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July 10, 2026Scientific ReportsOpen Access

Loss and resurgence of multi-drug-resistance during laboratory evolution of a carbapenem-resistant isolate of Escherichia coli

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Authors

THTaalin R. HøjABAllison BrowerAOAlexa Owens

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Overview

Randomized trial examines the changes in antibiotic resistance in Escherichia coli, indicating significant fluctuations over time.

Key Points

  • The research aims to understand how antibiotic resistance in a clinical isolate of Escherichia coli evolves under selective pressure.
  • An Escherichia coli isolate was cultured without antibiotics for 294 days.
  • A sub-inhibitory concentration of doripenem (DOR) was introduced from days 294-360 to observe resistance changes.
  • Whole-genome sequencing and RNAseq analysis were conducted to identify genetic and transcriptomic alterations.
  • DOR resistance increased by approximately 250-fold after selective pressure was applied.
  • Loss of bla NDM-1 occurred by Day 140, and bla CMY-6 by Day 319, while bla OXA-2 and bla TEM-1A persisted.
  • Changes in MICs to non-beta-lactam antibiotics were noted and will be investigated further.

Cite This Study

Høj et al. (2026) studied this question.

synapsesocial.com/papers/6a508b536eeac72a437a0003https://doi.org/10.1038/s41598-026-60112-w
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