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July 12, 2026Waste and Biomass ValorizationOpen Access

Activated Sludge for Aerobic Polyhydroxyalkanoate (PHA) Production with Acetate Supplement

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Authors

LLLei LiuTLTaina LundellMRMartin Romantschuk

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Overview

Randomized trial evaluates acetate-supplemented activated sludge for enhanced PHA production, indicating promising conditions for bioeconomic applications.

Key Points

  • This study aims to determine optimal conditions for polyhydroxyalkanoate (PHA) production using acetate-enriched activated sludge.
  • Acclimated activated sludge was collected from a full-scale wastewater treatment plant.
  • Laboratory-scale fermentation experiments (n = 5) compared fully aerobic operation with feast and famine strategies.
  • Microbial community composition was analyzed using shotgun metagenomic sequencing.
  • Fully aerobic conditions with acetate achieved 243.2 mg 3HB g −1 dry weight after 64 days of fermentation.
  • High PHA content was attained under stable aerobic conditions, surpassing conventional methods.
  • Microbial dominance included Pseudomonadota and Actinomycetota, with Xanthobacter as the primary PHA-producing genus.

Cite This Study

Liu et al. (2026) studied this question.

synapsesocial.com/papers/6a5332f94f7abc118adede1chttps://doi.org/10.1007/s12649-026-03592-1
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