A Rapid, Non-Destructive Technique for Microscopic Detection of Fungal Structures on Leaves and Fruit

This protocol describes a reproducible, non‑destructive method for quantifying spores and fungal structures on leaves and fruit surfaces using a modified nail‑polish imprint technique. The protocol is designed for use in laboratories or field‑associated diagnostic studies, enabling researchers to monitor the presence of pathogens over time without damaging the leaf and fruit surface. Step-by-step procedures for fruit and leaf selection, imprint preparation, staining, and microscopic evaluation to detect fungal structures, using inexpensive materials and readily available equipment are presented. This simple, adaptable approach to recovering fungal structures was designed to support studies in pathogen biology, seasonal epidemiology, and disease monitoring. Also, it can be incorporated into research, teaching, or extension applications that require accurate assessment of surface-colonizing fungi.