Circulating miR-21-5p was significantly elevated in acute myocardial infarction patients compared to healthy controls and demonstrated strong discriminatory accuracy with an AUC of 0.88.
Cross-Sectional (n=90)
Double-blind
No
Do circulating miR-16-5p and miR-21-5p discriminate AMI from healthy controls and correlate with early ventricular function and clinical stability in the early post-acute interval?
Circulating miR-21-5p and miR-16-5p are elevated 24-48 hours post-AMI and discriminate patients from controls, but do not correlate with early LVEF or clinical stability.
Effect estimate: AUC 0.88 (95% CI 0.80-0.95)
Absolute Event Rate: 36.19% vs 1.3%
p-value: p=<0.001
Abstract Background microRNAs show dynamic responses after acute myocardial infarction (AMI). The early post-acute interval (24–48 h after symptom onset) represents a transition from acute injury to early inflammatory signaling and constitutes a clinically relevant interval in which ventricular function and clinical stability are commonly reassessed. This study evaluated the discriminatory profile of circulating miR-16-5p and miR-21-5p during the early post-acute interval and examined their relationship with early ventricular function and clinical stability. Methods This cross-sectional study included 60 AMI patients and 30 healthy controls. Plasma miR-16-5p and miR-21-5p were quantified during 24–48 h post AMI using qRT-PCR and normalized to miR-103 as the endogenous control. Relative expression was calculated using the 2^–ΔΔCt method. LVEF and clinical stability were assessed during the same interval. Results Both miR-16-5p and miR-21-5p were significantly elevated in AMI patients compared with controls (p = 0. 003 and p 0. 05). Conclusion miR-16-5p and miR-21-5p are significantly elevated during the early post-acute interval of AMI, reflecting a distinct phase-specific molecular profile. miR-21-5p showed stronger discriminatory differences than miR-16-5p, indicating a more robust molecular signal during this phase. The absence of associations with LVEF or short-term clinical status suggests that these microRNAs reflect ongoing molecular activity rather than early functional impairment, with limited utility for early clinical risk assessment.
Dimitry et al. (Mon,) conducted a cross-sectional in Acute myocardial infarction (n=90). Circulating miR-16-5p and miR-21-5p vs. Healthy controls was evaluated on Circulating miR-21-5p levels and discriminatory accuracy for acute myocardial infarction (AUC 0.88, 95% CI 0.80-0.95, p=<0.001). Circulating miR-21-5p was significantly elevated in acute myocardial infarction patients compared to healthy controls and demonstrated strong discriminatory accuracy with an AUC of 0.88.