Cremastra appendiculata (D. Don) Makino, an endangered terrestrial orchid with significant medicinal value, lacks comprehensive genomic resources to elucidate its evolution and conservation genetics. Here, we present a chromosome-level genome assembly for C. appendiculata , generated by integrating PacBio circular consensus sequencing and Hi-C technologies. The final genome assembly totals 2.40 Gb, achieving contig and scaffold N50 values of 27.47 Mb and 98.06 Mb, respectively. Hi-C scaffolding anchored 98.66% of the assembly to 24 chromosomes, with repetitive elements comprising 83.85% of the genome and a total of 31,703 protein-coding genes predicted, of which 29,094 (91.77%) were functionally annotated. This high-quality, chromosome-level reference genome provides a foundational resource for understanding the population genetic structure, adaptive evolution and speciation mechanisms of C. appendiculata , thereby offering valuable insights into its evolutionary history and conservation.
Tang et al. (Mon,) studied this question.
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