Frontispiece: Allosteric Regulation of Photophysics and Binding in Oxazine‐macrocycle Complexes at Single‐molecule Resolution

In Research Article e1546846, Thorben Cordes and co-workers examine how the oxazine dye ATTO655 interacts with two macrocyclic hosts using single molecule optical spectroscopy. ATTO655 forms an inclusion complex with cucurbit[8]uril, whereas p sulfonatocalix[4]arene produces dim exclusion complexes. Choline can bind simultaneously, creating a ternary system that modulates affinity and suppresses photoinduced dark states, demonstrating allosteric control of dye binding and photophysics relevant for sensing and bioimaging applications.