AVITHRAPID - Standard Operating Procedure - PLpro constructs protein production

1.1. PLproThis Standard Operating Procedure (SOP) describes the standardized method for the SARS-CoV-2 Papain-like protease (PLpro, corresponding to 1564–1878 of nsp3) recombinant protein production. The purified protein is used for biochemical, biophysical and structural evaluation of inhibitors within the AVITHRAPID project.1.2. PLpro60-315This Standard Operating Procedure (SOP) describes the standardized method for the SARS-CoV-2 Papain-like protease deleted of Ubl domain (PLpro60-315, corresponding to 1624–1878 of nsp3) recombinant protein production. The purified protein is used for biophysical and structural evaluation of inhibitors within the AVITHRAPID project.1.3. PLpro-NABThis Standard Operating Procedure (SOP) describes the standardized method for the SARS-CoV-2 Papain-like protease-Nucleic Acid Binding domain (PLpro-NAB, corresponding to 1564–2047 of nsp3) recombinant protein production. The purified protein was used for biochemical and biophysical evaluation of inhibitors within the AVITHRAPID project.