Randomized trial shows miR-766-3p as a potential diagnostic and prognostic biomarker in children, indicating critical inflammation regulation.
Key Points
This research aims to explore the clinical value and molecular mechanisms of miR-766-3p in severe pneumonia in children.
Detected miR-766-3p levels in plasma using quantitative real-time PCR (RT-qPCR).
Assess diagnostic performance with receiver operating characteristic (ROC) curves and prognostic value with Kaplan–Meier (KM) curves and Cox regression models.
Mimicked pneumonia environment in vitro using lipopolysaccharide (LPS), measuring cell viability and inflammatory factors.
miR-766-3p levels were reduced by 0.36-fold in children with severe pneumonia compared to healthy children.
Patients with low miR-766-3p expression had a lower accumulative survival rate than those with high expression (average level ≤ 0.65 vs. > 0.65).
Cox regression indicated miR-766-3p as an independent risk factor for poor outcomes, reducing inflammation induced by LPS.