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The metabolism and the in vitro effects of the cytokinin N 6 ‐ (3‐hydroxybenzyl) adenine (meta‐topolin, mT) were compared with those of N 6 ‐benzyladenine (BA), N 6 ‐benzyl‐9‐ (2‐tetrahydropyranyl) adenine (BPA) and N 6 ‐benzyladenine‐9‐riboside (9RBA) in micropropagated Spathiphyllum floribundum Schott cv. Petite. In vitro, BA, BPA and 9RBA were mainly converted into the stable derivative N 6 ‐benzyladenine‐9‐glucoside (9GBA), located at the basal part of the plant. N 6 ‐ (3‐O‐β‐D‐glucopyranosyl) benzyladenine‐9‐riboside (OG) 9RmT was the main derivative of mT. This new cytokinin‐O‐glucoside, which was present in all plant parts, was metabolized much faster than 9GBA during acclimatization. The effect of BA and mT on in vitro shoot and root production and post vitro rooting was compared. Only mT combined a good shoot production with in vitro root formation. The plants that developed on medium with 10 µ M or more mT rooted better during acclimatization than those developed on medium with equimolar concentrations of BA.
Werbrouck et al. (Tue,) studied this question.