January 30, 2016Journal of Biological ChemistryOpen Access

Differential Roles of Protein Complexes NOX1-NOXO1 and NOX2-p47phox in Mediating Endothelial Redox Responses to Oscillatory and Unidirectional Laminar Shear Stress

Q: What is the clinical evidence from this study?

Study design: Other. Population: Endothelial redox responses. Intervention: Laminar shear stress (LSS) and Oscillatory shear stress (OSS). Primary outcome: Superoxide and NO production, eNOS phosphorylation.

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Key result

Laminar shear stress activates the NOX2-p47phox complex to promote NO production, whereas oscillatory shear stress activates the NOX1-NOXO1 complex to uncouple eNOS and increase superoxide.

Population

Endothelial cells in an in vitro model using a software-controlled, valve-operated OsciFlow device with…

Comparison

Application of unidirectional laminar shear… vs Resting state or control conditions without…

Design

Preclinical

Authors

KSKin Lung SiuUniversity of California, Los Angeles LGLing GaoKing's College London HCHua CaiUniversity of California, Los Angeles

Discussion

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Implication

Hypothesis-generating for shear stress effects on endothelial superoxide; leaves open any clinical role in atherosclerosis.

Structured PICO

Population

Endothelial cells in an in vitro model using a software-controlled, valve-operated OsciFlow device with parallel chambers

Intervention

Application of unidirectional laminar shear stress (LSS) and atherogenic oscillatory shear stress (OSS), combined with RNA interference (RNAi) of p47phox, NOX2, and NOXO1, or PEG-catalase

Comparator

Resting state or control conditions without specific RNAi/shear stress

Outcome

Superoxide production, nitric oxide (NO) production, and endothelial nitric oxide synthase (eNOS) phosphorylation/uncouplingsurrogate

This study demonstrates that different shear stresses activate distinct NADPH oxidase complexes in endothelial cells, providing a mechanistic basis for the vasoprotective effects of laminar flow and the atherogenic effects of oscillatory flow.

Cite This Study

Siu et al. (2016) studied Endothelial redox responses. Laminar shear stress (LSS) and Oscillatory shear stress (OSS) was evaluated on Superoxide and NO production, eNOS phosphorylation. Laminar shear stress activates the NOX2-p47phox complex to promote NO production, whereas oscillatory shear stress activates the NOX1-NOXO1 complex to uncouple eNOS and increase superoxide.

synapsesocial.com/papers/6a5bcea6ec0f450dc43ae77d https://doi.org/10.1074/jbc.m115.713149

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Differential Roles of Protein Complexes NOX1-NOXO1 and NOX2-p47phox in Mediating Endothelial Redox Responses to Oscillatory and Unidirectional Laminar Shear Stress

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Differential Roles of Protein Complexes NOX1-NOXO1 and NOX2-p47phox in Mediating Endothelial Redox Responses to Oscillatory and Unidirectional Laminar Shear Stress

Discussion

Member takes

Implication

Structured PICO

Cite This Study

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