Five fluorescent dyes (BASO Red 546, Brilliant Sulfaflavine, Fluorescein, Uvitex OB, and Rhodamine B) andseven nonfluorescent dyes (Amaranth, Reactive Black 5, Patent Blue Violet, Tartrazine, Lissamine Green B, Erythrosin B,and Ponceau 4R) were tested to determine their potential in tracer application for spray deposit assessment. Laboratoryexperiments were performed to determine the tracer recovery rate from paper targets, either kept in the dark or exposed tosunlight. Tartrazine was easily extractable and relatively photostable and was chosen for further evaluation on differenttargets (paper, Mylar, and vine leaves), either sprayed or manually treated. Tartrazine was considered suitable for depositassessment from agricultural sprayers because of no toxicity, simple laboratory procedure, high recovery rate, and relativelylow photodegradation. However, field experiments should be properly planned to reduce, or to account for, variability ofnatural targets (variable extraction rate and possible background deposits) and photodegradation under exposure to sunlight.
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Gianfranco Pergher (2001) studied this question.