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Recent articles by Wagner et al[1][1] and Hochegger et al[2][2] reported the expression of perforin and granzymes A and B in human polymorphonuclear cells (PMNs; neutrophils) using flow cytometry assays. Both groups used an intracellular staining technique, including the use of secondary antibodies
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Grossman et al. (2004) studied this question.
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